Proteine

Inhalt, Gliederung

Vorlesungsskripten und Vorlesungsmaterialien

Kovalente Struktur von Proteinen und Nukleinsäure
Vorlesungsskript - Format: PDF

Pflanzliche Proteine
Einiges über pflanzliche Proteine. Botanik online

Proteinanalytik
... mit 2-D PAGE - Format: PDF

Proteinbiosynthese, Translation
Grundlagen. Botanik online

Proteinchemie
Einführung in die Proteinchemie: Schematische und sehr kurze Einführung in die grundlegenden Mechanismen der Proteinchemie

Proteinchemie
Vorlesungsmaterialien Chemische Biologie: Peptid- und Proteinchemie. Universität Leipzig

Proteine
Grundlagen: Aminosäuren, Peptide, Sekundärstrukturen, Tertiärstrukturen; Botanik online

Proteine: Entstehung, abiotische Bedingungen
Können unter abiotischen Bedingungen auch Makromoleküle entstehen?. Botanik online

Proteinreinigung
Vorlesungsskript. Universität Leipzig - Format: PDF

Proteinstruktur
Einführung in die räumliche Struktur von Proteinen - Format: PDF

Proteinstruktur
Graphische Darstellung von Proteinstrukturen. Botanik online

Proteinstruktur
Einführung in die räumliche Struktur von Proteinen. FH Merseburg - Format: PDF

Proteinstruktur
... und Modellierung - Format: PDF

Struktur und Faltung von Proteinen
Vorlesungsmaterialien. Universität Halle-Wittenberg - Format: PDF

Spezielle Teilinformationen

Proteine als Sonden
Proteine als analytische Hilfsmittel zur Lokalisation von Molekülen und zum Studium der molekularen Architektur der Zellen. Botanik online

Proteine von Menschenhand
Wie sich mit "Protein Engineering" ohne Tiere Antikörper gewinnen lassen. Universität Zürich

Hilfsmittel und Daten für Labor und Praktikum

Proteinreinigung
Reinigung rekombinanter Proteine. Universität Frankfurt - Format: PDF

Proteinreinigung
Techniken der Protein-Reinigung - Format: PDF

Proteinreinigung
Grundlagen, Techniken. Springer - Format: PDF

Praktikumsskripten, praktische Anleitungen

Biochemisches Praktikum
Sammlung mit Praktikumsversuchen - Format: PDF

ELISA
Immunologischer Nachweis - Format: PDF

Funktion der Proteine Ebony und Tan
Die Funktion der Proteine Ebony und Tan im visuellen System von Drosophila melanogaster - Format: PDF

Immuno-Affinitätschromatographie
Antikörper in der Molekularbiologie; Theorie. SwissEduc - Format: PDF

Proteinbestimmung mit Biuret und Folin Proteinbestimmung mit Biuret und Folin
... nach enzymatischer Spaltung von Casein - Format: PDF

Proteine
Praktikumsskript. Universität Giessen - Format: PDF

Proteinreinigung
Anleitungen - Format: PDF

Proteinreinigung
Praktikumsskript: Proteinbiochemische Methoden. TU Freiberg - Format: PDF

Proteinreinigung und Charakterisierung
Prinzipien - Format: PDF

Ultrafiltration
Versuchsprotokoll - Format: PDF

Western-Blotting
Praktikumsskript - Format: PDF

Metabolismus, Stoffwechselwege

Stoffwechsel
... von Proteinen und Aminosäuren - Format: PDF

Software und Programme

AMBER
... refers to two things: a set of molecular mechanical force fields for the simulation of biomolecules (which are in the public domain, and are used in a variety of simulation programs); and a package of molecular simulation programs which includes source code and demos - [engl.]

CLC Combined Workbench
... creates a software environment enabling users to make a large number of advanced protein, DNA, and RNA sequence analyses, combined with smooth data management, and excellent graphical viewing and output options - [engl.]

EMBOSS
... is a free Open Source software analysis package specially developed for the needs of the molecular biology (e.g. EMBnet) user community. The software automatically copes with data in a variety of formats and even allows transparent retrieval of sequence data from the web. Also, as extensive libraries are provided with the package, it is a platform to allow other scientists to develop and release software in true open source spirit. EMBOSS also integrates a range of currently available packages and tools for sequence analysis into a seamless whole. EMBOSS breaks the historical trend towards commercial software packages - [engl.]

ENDscript
... is an interface grouping eight types of programs: BLAST; MULTALIN/ CLUSTALW; DSSP; CNS; ESPript; BOBSCRIPT/ MOLSCRIPT; DINO; PHYLODENDRON; PROFIT. It aims to produce from a single PDB file four PostScript figures containing most required sequence and structure information on a protein of known structure - [engl.]

ESPript
ESPript, Easy Sequencing in Postscript, is a utility to generate a pretty PostScript output from aligned sequences - [engl.]

ExPASy Proteomics Server
The ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics (SIB) is dedicated to the analysis of protein sequences and structures as well as 2-D PAGE - [engl.]

ExPASy Tools
ExPASy Proteomics tools - [engl.]

iProClass
The iProClass database provides value-added information reports for UniProtKB and unique NCBI Entrez protein sequences in UniParc, with links to over 90 biological databases, including databases for protein families, functions and pathways, interactions, structures and structural classifications, genes and genomes, ontologies, literature, and taxonomy - [engl.]

iProLINK
The Integrated Protein Literature Information and Knowledge has been developed as a resource to facilitate text mining in the area of literature-based database curation, named entity recognition, and protein ontology development - [engl.]

IRECS
... is a program that predicts the conformation of protein side chains. It does so either using only backbone information or using additional template information about the side-chain conformations - [engl.]

MolScript
... is a program for displaying molecular 3D structures, such as proteins, in both schematic and detailed representations - [engl.]

OpenRasMol
Open Source versions of RasMol - [engl.]

PIRSF
The PIRSF concept is being used as a guiding principle to provide comprehensive and non-overlapping clustering of UniProtKB sequences into a hierarchical order to reflect their evolutionary relationships. The PIRSF classification system is based on whole proteins rather than on the component domains; therefore, it allows annotation of generic biochemical and specific biological functions, as well as classification of proteins without well-defined domains - [engl.]

Protein Family Databases
Link collection - [engl.]

Protein Software
Download-Seite, ZDNET - [engl.]

Protein Software
Download - Seite - [engl.]

Proteome - Scaffold Software
Confident Protein Identification: Scaffold software works with SEQUEST® or Mascot® to identify proteins from mass spectrometry data. Scaffold lets you validate the mass spec results both statistically and visually - [engl.]

PSORT
... provides links to the PSORT family of programs for subcellular localization prediction as well as other datasets and resources relevant to localization prediction - [engl.]

RasMol
... is a program for molecular graphics visualisation - [engl.]

Software Liste, Sammlung
The RCSB PDB provides a variety of tools and resources for studying the structures of biological macromolecules and their relationships to sequence, function, and disease - [engl.]

Swiss-PdbViewer
DeepView - Swiss-PdbViewer is an application that provides a user friendly interface allowing to analyze several proteins at the same time. The proteins can be superimposed in order to deduce structural alignments and compare their active sites or any other relevant parts. Amino acid mutations, H-bonds, angles and distances between atoms are easy to obtain thanks to the intuitive graphic and menu interface - [engl.]

UniProt
Universal Protein Resource is the world's most comprehensive catalog of information on proteins - [engl.]

Journale, Fachzeitschriften

Current Protein and Peptide Science
The purpose of Current Protein & Peptide Science is to present summaries of specific aspects of research involving proteins, peptides, and interactions between the enzymes, especially proteases; the binding interactions of hormones and their receptors; the properties of transcription factors and other molecules that regulate gene expression; the reactions leading to the immune response; the process of signal transduction; the structure and function of proteins involved in the cytoskeleton and molecular motors; the properties of membrane channels and transporters; and the generation and storage of metabolic energy. Bentham - [engl.]

Current Proteomics
... publishes review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, softwares, databases, technological advances and applications of proteomics, including functional proteomics. Bentham - [engl.]

EMBO Journal
... provides for rapid publication of full-length papers describing original research of general rather than specialist interest in molecular biology and related areas - [engl.]

Peptides
... is an international journal presenting original contributions on the chemistry, biochemistry, neurochemistry, endocrinology, gastroenterology, physiology, and pharmacology of peptides, as well as their neurological, psychological and behavioral effects. Elsevier - [engl.]

Protein and Peptide Letters
... publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Bentham - [engl.]

Protein Engineering Design and Selection
PEDS publishes research papers and review articles relevant to the engineering, design and selection of proteins for use in biotechnology and therapy, and for understanding fundamental properties of activity, stability, folding, misfolding and disease. Oxford University Press - [engl.]

Protein Expression and Purification
... is dedicated to providing a forum for information about protein isolation based on conventional fractionation as well as techniques employing various molecular biological procedures to increase protein expression. Elsevier - [engl.]

Protein Science
... serves as an international forum for publishing original reports on proteins in the broadest sense. The Journal aims to unify this field by cutting across established disciplinary lines and focusing on “protein-centered” science. The Protein Society. Wiley - [engl.]

Proteins: Structure, Function, and Bioinformatics
... publishes original reports of significant experimental and analytic research in all areas of protein research: structure, function, computation, genetics, and design. Wiley-Interscience. Wiley - [engl.]

Proteomics
... is the premier international source for information on all aspects of applications and technologies in proteomics. Wiley-Interscience. Wiley - [engl.]

Regulatory Peptides
... provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Elsevier. Elsevier - [engl.]

Dissertationen

Modellierung der Tertiärstrukturen lysosomaler Cysteinproteasen
Charakterisierung der katalytisch aktiven Bindungszentren und strukturbasiertes Ligandendesign; Online-Dissertation, 2000, Uni Halle

Proteinstruktur und Proteinentfaltungsanalysen ...
... mit Hilfe der Ramanspektroskopie: Untersuchung an Modellpeptiden zur Optimierung der Methodik. Dissertation, PDF-Format, Universität Bremen, 2000

Newsarchiv

Der Terahertz-Tanz des Wassers mit den Proteinen
Protein-Einfluss hat eine unvermutet große Reichweite. Es ist besonders die Art der Faltung, die bei Proteinen ihre Funktion bestimmt - ein dynamischer Prozess, der sehr schnell abläuft. Bei der Untersuchung dieses "Tanzes" der Proteine hat man bislang den Partner außer Acht gelassen: das Wasser. Dieses Zusammenspiel zwischen Wasser und Proteinen haben Forscher aus Bochum, Illinois und Nevada um Prof. Dr. Martina Havenith-Newen (Physikalische Chemie II der RUB) nun mittels Terahertz-Spektroskopie beobachtet.

Magnetische Effekte in Eisen-Schwefel-Proteinen
Eiweißmoleküle, die kleine magnetische Zentren aus Eisenatomen enthalten, spielen eine wichtige Rolle beim Energiefluss in lebenden Organismen. Forscher um Prof. Dr. Dominik Marx (Lehrstuhl für Theoretische Chemie der Ruhr-Universität) haben nun erstmals gezeigt, dass die magnetischen Eigenschaften dieser Zentren eng an die Dynamik der Proteinumgebung gekoppelt sind. Diese aufgrund einer neuen mehrskaligen Simulationstechnik gewonnenen Ergebnisse wurden nun in den renommierten "Proceedings of the National Academy of Sciences" (PNAS) publiziert.

Neues Verfahren vermisst aktive Zentren von Proteinen
Mit einer speziellen Methode für gepulste EPR-Spektroskopie bei hohen Magnetfeldern gelang es Wissenschaftlern erstmals, die genaue Orientierung der aktiven Zentren eines Proteinkomplexes zu vermessen.

RUB-Chemiker "verfilmen" die Proteinfaltung
Neue KITA-Spektroskopie erlaubt Echtzeit-Beobachtung. Artikel, August 2008.
Bild [Quelle: RUB, Wiley]: Die kinetische Terahertz-Absorption (KITA) verwendet Pikosekundenpulse, um Solvatationsdynamiken während Selbstorganisationsprozessen auf der Zeitskala zwischen Millisekunden bis Sekunden zu erfassen. Die Anwendung der Methode zur Untersuchung der Umorganisation des Wassersolvats bei der Faltung eines Proteins (hier: Ubiquitin) beschreiben M. Gruebele et al. in der Angewandte Chemie.

Institute und Forschungseinrichtungen

Halle-Wittenberg
Arbeitsgruppe Proteintechnologie an der Martin-Luther-Universität Halle-Wittenberg - [engl.]

UltraStrukturNetzwerk
USN ist ein Projekt, das durch das MPI für Molekulare Genetik in Kooperation mit der Charité 2002 initiiert wurde.